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         | Research > Research Cores > Hybridoma Center The Washington University School of Medicine Hybridoma Center (WUHC) was established in 1979 to provide monoclonal antibody technology to the University research community. The Hybridoma Center assists investigators in the generation of monoclonal antibody-producing B cell hybridomas. Initially, guidance is provided by the Director in the selection of appropriate species to be used, development of immunization schedules, screening protocols, and methods for antibody production. Immunized spleen cells derived from either mice, Armenian hamsters, or rats are fused and maintained at the Hybridoma Center. Once sufficient growth has occurred, culture supernatants are provided to the investigator for screening. Hybridomas secreting antigen-specific antibodies are cloned to establish a stable line secreting a unique antibody with defined specificity and isotype. After a second round of cloning, the antibody producing cultures are expanded and returned to the investigator for further processing. Frozen vials are maintained in liquid nitrogen by the Hybridoma Center. The WUHC performs nearly 100 fusions each year, providing an essential and cost-effective service to members of the research community. Hybridoma Center Guidelines Antibody Resource Links ABCAM For more information: Director: Kathleen Sheehan, PhD | |
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